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Antibodies > NFYA > LS-C352582
 
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Catalog Number Size Price
LS-C352582-100 100 µl (1 mg/ml) $299 
NFYA Antibody - Immunohistochemical analysis of NFYA staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NFYA Antibody - Immunofluorescent analysis of NFYA staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
NFYA Antibody - Western blot analysis of NFYA expression in A431 (A); K562 (B); NIH3T3 (C) whole cell lysates.
NFYA Antibody - Immunohistochemical analysis of NFYA staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NFYA Antibody - Immunofluorescent analysis of NFYA staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
NFYA Antibody - Western blot analysis of NFYA expression in A431 (A); K562 (B); NIH3T3 (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human NFYA Antibody (C‑Terminus, IHC, IF, WB) LS‑C352582

Polyclonal Rabbit anti‑Human NFYA Antibody (C‑Terminus, IHC, IF, WB) LS‑C352582

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Antibody:
NFYA Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352582-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NFYA Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
NFYA antibody LS-C352582 is an unconjugated rabbit polyclonal antibody to NFYA (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB.
Target
Human NFYA
Synonyms
NFYA | CBF-A | CBF-B | HAP2 | HAP2 CCAAT-binding protein | NF-YA
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human NFYA.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of NFYA protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFYA
P23511 NM_021705 NP_068351.1

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Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA, Gel shift
NFYA Antibody - Detection of Human and Mouse NF-YA by Immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse hybridoma tumor (right). Antibody: Affinity purified rabbit anti-NF-YA used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
NFYA Antibody (aa333‑347) LS‑C288861
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Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
NFYA Antibody - NFYA antibody ARP31263_T100-NP_002496-NFYA (nuclear transcription factor Y, alpha) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human liver. This image was taken for the unconjugated form of this product. Other forms have not been tested.
NFYA Antibody (C‑Terminus) LS‑C31566
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
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Applications: IHC, Immunofluorescence, Western blot, ELISA
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See all NFYA Antibodies

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/27/2024
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