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Catalog Number Size Price
LS-C801193-100 100 µl (1 mg/ml) $379 
LS-C801193-200 200 µl (1 mg/ml) $421 
NFKBIB / IKB Beta / IKBB Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
NFKBIB / IKB Beta / IKBB Antibody - Western blot analysis of IkappaB-beta phosphorylation expression in TNF-a treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
NFKBIB / IKB Beta / IKBB Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
NFKBIB / IKB Beta / IKBB Antibody - Western blot analysis of IkappaB-beta phosphorylation expression in TNF-a treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human NFKBIB / IKB Beta / IKBB Antibody (phospho‑Thr19, IF, WB) LS‑C801193

Polyclonal Rabbit anti‑Human NFKBIB / IKB Beta / IKBB Antibody (phospho‑Thr19, IF, WB) LS‑C801193

Antibody:
NFKBIB / IKB Beta / IKBB Rabbit anti-Human Polyclonal (pThr19) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801193-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NFKBIB / IKB Beta / IKBB Rabbit anti-Human Polyclonal (pThr19) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IKBB antibody LS-C801193 is an unconjugated rabbit polyclonal antibody to human IKBB (NFKBIB / IKB Beta) (pThr19). Validated for IF, Peptide-ELISA and WB.
Target
Human NFKBIB / IKB Beta / IKBB
Synonyms
NFKBIB | IKBB | I-kappa-B-beta | IkappaBbeta | NF-kappa-B inhibitor beta | IkB-beta | TRIP9 | TRIP-9 | IkB-B | NF-kappa-BIB | TR-interacting protein 9
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human I kappaB- beta around the phosphorylation site of Threonine 19.
Epitope
pThr19
Specificity
Phospho-I kappaB- beta (Thr19) Antibody detects endogenous levels of I kappaB- beta only when phosphorylated at Threonine 19.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFKBIB / IKB Beta / IKBB
Q15653 NM_002503 NP_002494.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/31/2024