LSBio logo with text
Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers


Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > NFKB1 / NF-Kappa-B > LS-C801049
 
Print Page  
Catalog Number Size Price
LS-C801049-100 100 µl (1 mg/ml) $379 
LS-C801049-200 200 µl (1 mg/ml) $421 
NFKB1 / NF-Kappa-B Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NFKB1 / NF-Kappa-B Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
NFKB1 / NF-Kappa-B Antibody - Staining HeLa cells treated with EGF 200nM 5' cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
NFKB1 / NF-Kappa-B Antibody - Western blot analysis of NF-kappaB p105/p50 phosphorylation expression in LPS treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
NFKB1 / NF-Kappa-B Antibody - Western blot analysis of Phospho-NF-kappaB p105/p50 (Ser927) expression in various lysates
NFKB1 / NF-Kappa-B Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
NFKB1 / NF-Kappa-B Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
NFKB1 / NF-Kappa-B Antibody - Staining HeLa cells treated with EGF 200nM 5' cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
NFKB1 / NF-Kappa-B Antibody - Western blot analysis of NF-kappaB p105/p50 phosphorylation expression in LPS treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
NFKB1 / NF-Kappa-B Antibody - Western blot analysis of Phospho-NF-kappaB p105/p50 (Ser927) expression in various lysates
1 of 5
2 of 5
3 of 5
4 of 5
5 of 5

Polyclonal Rabbit anti‑Human NFKB1 / NF‑Kappa‑B Antibody (phospho‑Ser927, IHC, IF, WB) LS‑C801049

Polyclonal Rabbit anti‑Human NFKB1 / NF‑Kappa‑B Antibody (phospho‑Ser927, IHC, IF, WB) LS‑C801049

Submit Review LSBio Guarantee
Antibody:
NFKB1 / NF-Kappa-B Rabbit anti-Human Polyclonal (pSer927) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801049-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NFKB1 / NF-Kappa-B Rabbit anti-Human Polyclonal (pSer927) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NF-Kappa-B antibody LS-C801049 is an unconjugated rabbit polyclonal antibody to NF-Kappa-B (NFKB1) (pSer927) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human NFKB1 / NF-Kappa-B
Synonyms
NFKB1 | Ebp- 1 | EBP-1 | NF-KappaB p50 | NFkappaB | NFKB-p50 | KBF1 | NF-kappa-B | NF-kappaB | NF-kB1 | NFKB-p105 | p105 | p50 | DNA binding factor KBF1 | DNA-binding factor KBF1 | NF-kappabeta
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human NF- kappaB p105/p50 around the phosphorylation site of Serine 927.
Epitope
pSer927
Specificity
Phospho-NF- kappaB p105/p50 (Ser927) Antibody detects endogenous levels of NF- kappaB p105/p50 only when phosphorylated at Serine 927.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFKB1 / NF-Kappa-B
P19838 NM_003998 NP_003989.2

Publications (0)

Submit a Publication for LS-C801049

Customer Reviews (0)

Submit a review for LS-C801049

Featured Products

NFKB1 / NF-Kappa-B Antibody - Anti-NF-KappaB p105 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
NFKB1 / NF‑Kappa‑B Antibody (N‑Terminus) LS‑B8072
Species: Human, Chicken
Applications: IHC, IHC - Paraffin, Western blot
NFKB1 / NF-Kappa-B Antibody - Anti-NFKB1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
IHC‑plus™ NFKB1 / NF‑Kappa‑B Antibody (clone 4D1) LS‑B1668
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
NFKB1 / NF-Kappa-B Antibody - NFKB1 antibody Western blot of 1 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
NFKB1 / NF‑Kappa‑B Antibody (C‑Terminus) LS‑C112312
Species: Human
Applications: Western blot
NFKB1 / NF-Kappa-B Antibody - Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]
NFKB1 / NF‑Kappa‑B Antibody (clone 3F6) LS‑C133346
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
NFKB1 / NF-Kappa-B Antibody - Anti-NFKB1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
IHC‑plus™ NFKB1 / NF‑Kappa‑B Antibody (N‑Terminus) LS‑B395
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Product
NFKB1 / NF‑Kappa‑B Antibody (Biotin) LS‑C528248
Species: Human
Applications: Western blot, ELISA
See all NFKB1 / NF-Kappa-B Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/24/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2024 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

To place an order:
customerservice@vectorlabs.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com