Target
Human Neurofascin / NF
Synonyms
NFASC | Neurofascin homolog | NF | KIAA0756 | Neurofascin | Neurofascin homolog (chicken) | NRCAML
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-NFSPNQTKFTVQRT, from the internal region of the protein sequence according to NP_001005388.2NP_001153803.1NP_001153804.1NP_055905.2;.
Specificity
Human NFASC / Neurofascin. This antibody is expected to recognize reported isoforms 1, 2, 3 and 4 (NP_001005388.2;,NP_001153803.1, NP_001153804.1and NP_055905.2 resp) .
Applications
- Western blot (0.5 - 2 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 170kD band observed in Human Lung and Rat Brain lysates (calculated MW of 138kD according to NP_001005388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kirschbaum et al, J Biol Chem. 2009 Oct 16;284(42):28533-42. PMID: 19666467). Recommended concentration: 0.5-2 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee