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Catalog Number	Size	Price
LS-C97685-400	400 µl	$393

NES / Nestin Antibody - Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with Nestin Antibody (S1409), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

NES / Nestin Antibody - Fluorescent confocal image of SY5Y cells stained with Nestin (S1409) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated Nestin (S1409) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Nestin (S1409) immunosignal to the intermediate filaments, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000132688).

NES / Nestin Antibody - Western blot of Nestin Antibody (S1409) in HepG2 cell line lysates (35 ug/lane). NES (arrow) was detected using the purified antibody.

NES / Nestin Antibody - Flow cytometric of HepG2 cells using Nestin Antibody (S1409)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Polyclonal Rabbit anti‑Human NES / Nestin Antibody (Ser1409, IHC, IF, WB) LS‑C97685

Name: Anti-NES / Nestin Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C97685-400
Price: 393.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

(4.0) Rating

Submit Review (1) Publication(s) LSBio Guarantee

Antibody:

NES / Nestin Rabbit anti-Human Polyclonal (Ser1409) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

$393

LS-C97685-400

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

NES / Nestin Rabbit anti-Human Polyclonal (Ser1409) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

Nestin antibody LS-C97685 is an unconjugated rabbit polyclonal antibody to human Nestin (NES) (Ser1409). Validated for Flow, IF, IHC and WB. Cited in 1 publication.

(1) Publication(s)

Target

Human NES / Nestin

Synonyms

NES | Nestin | Nbla00170

See All NES / Nestin Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Protein A purified

Modifications

Unmodified

Epitope

Ser1409

Specificity

This Nestin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1389-1416 amino acids from human Nestin.

Applications

IHC
IHC - Paraffin (1:50 - 1:100)
Immunofluorescence (1:200)
Western blot (1:1000)
Flow Cytometry (1:10 - 1:50)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About NES / Nestin

Uniprot: P48681 NCBI: X65964 CAA46780.1

LSBio Ratings

NES / Nestin Antibody (Ser1409) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-C97685 has an LSBio Rating of

(4.0) Rating

Publications (4)

Learn more about The LSBio Ratings Algorithm

Publications (1)

Identification of a novel putative pancreatic stem/progenitor cell marker DCAMKL-1 in normal mouse pancreas. May R, Sureban SM, Lightfoot SA, Hoskins AB, Brackett DJ, Postier RG, Ramanujam R, Rao CV, Wyche JH, Anant S, Houchen CW. American journal of physiology. Gastrointestinal and liver physiology. 2010 299:G303-10.

PubMed: 20522640 PMC: PMC2928534

Submit a Publication for LS-C97685