Target
Human NCK1 / NCK
Synonyms
NCK1 | Cytoplasmic protein nck | Cytoplasmic protein NCK1 | NCK adaptor protein 1 | Nck-1 | NCKalpha | Melanoma NCK protein | NCK | NCK tyrosine kinase
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
Purified GST fusion protein corresponding to amino acids 1 to 254 of human Nck, produced in E. coli.
Specificity
Recognizes a human 47kD SH2/SH3-containing protein, Nck. Species cross-reactivity: Mouse and rat.
Applications
- Western blot (0.1 - 1 µg/ml)
- Immunoprecipitation
Usage
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using goat anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5 ug immunoprecipitates Nck from a lysate of human A431 carcinoma cells, mouse 3T3/A31 fibroblasts and rat L6 skeletal fibroblasts.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee