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Catalog Number Size Price
LS-C353699-30 30 µl $263 
LS-C353699-50 50 µl $279 
LS-C353699-100 100 µl (1 mg/ml) $315 
LS-C353699-200 200 µl $379 
NCF1 / p47phox / p47 phox Antibody - Immunofluorescent analysis of p47 phox (pS370) staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
NCF1 / p47phox / p47 phox Antibody - Western blot analysis of p47 phox (pS370) expression in HeLa serum starvation-treated (A); THP1 (B); Raw264.7 serum starvation-treated (C); H9C2 serum starvation-treated (D) whole cell lysates.
NCF1 / p47phox / p47 phox Antibody - Immunofluorescent analysis of p47 phox (pS370) staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
NCF1 / p47phox / p47 phox Antibody - Western blot analysis of p47 phox (pS370) expression in HeLa serum starvation-treated (A); THP1 (B); Raw264.7 serum starvation-treated (C); H9C2 serum starvation-treated (D) whole cell lysates.
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Polyclonal Rabbit anti‑Human NCF1 / p47phox / p47 phox Antibody (phospho‑Ser370, IF, WB) LS‑C353699

Polyclonal Rabbit anti‑Human NCF1 / p47phox / p47 phox Antibody (phospho‑Ser370, IF, WB) LS‑C353699

Antibody:
NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (pSer370) Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$263
LS-C353699-30
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (pSer370) Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
P47 phox antibody LS-C353699 is an unconjugated rabbit polyclonal antibody to p47 phox (NCF1 / p47phox) (pSer370) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IP and WB.
Target
Human NCF1 / p47phox / p47 phox
Synonyms
NCF1 | NCF1A | Neutrophil cytosolic factor 1 | NCF-47K | Nox organizer 2 | NOXO2 | p47-phox | p47phox | SH3PXD1A | NADPH oxidase organizer 2 | NCF-1 | Neutrophil cytosol factor 1 | Nox-organizing protein 2
Host
Rabbit
Reactivity
Human, Mouse, Rat, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human p47 phox.
Epitope
pSer370
Specificity
Recognizes endogenous levels of p47 phox (pS370) protein.
Applications
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NCF1 / p47phox / p47 phox
P14598 NM_000265 NP_000256.4

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 3/31/2025