Target
Human NCF1 / p47phox / p47 phox
Synonyms
NCF1 | NCF1A | Neutrophil cytosolic factor 1 | NCF-47K | Nox organizer 2 | NOXO2 | p47-phox | p47phox | SH3PXD1A | NADPH oxidase organizer 2 | NCF-1 | Neutrophil cytosol factor 1 | Nox-organizing protein 2
Reactivity
Human, Bovine
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
GST fusion protein corresponding to full-length human p47-phox.
Specificity
Recognizes human p47-phox, Mr 47kD. Species cross-reactivity: Reported cross-reactivity with bovine.
Applications
- ICC (1:250 - 1:2000)
- Western blot (1:250 - 1:1000)
- Immunoprecipitation
Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:250-1:1000 detected p47-phox in RIPA lysates from HL-60 cells. Immunoprecipitation: 10 ul immunoprecipitates p47-phox from 500 ug of HL-60 RIPA lysate. Immunocytochemistry: 1:250-1:2000 dilution showed positive cytosolic immunostaining for p47-phox in HL-60 cells fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
Presentation
5 mM EDTA, 0.02% sodium azide, 40% glycerol.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NCF1 / p47phox / p47 phox