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Customer Service: (800) 227-6666 / (650) 697-3600


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Catalog Number Size Price
LS-C368438-30 30 µl (1 mg/ml) $255 
LS-C368438-100 100 µl (1 mg/ml) $299 
LS-C368438-200 200 µl (1 mg/ml) $351 
NCF1 / p47phox / p47 phox Antibody - Immunohistochemical analysis of p47 phox staining in human lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NCF1 / p47phox / p47 phox Antibody - Immunofluorescent analysis of p47 phox staining in Raji cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
NCF1 / p47phox / p47 phox Antibody - Western blot analysis of p47 phox expression in Raji (A); Ramos (B); Raw264.7 (C) whole cell lysates.
NCF1 / p47phox / p47 phox Antibody - Immunohistochemical analysis of p47 phox staining in human lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NCF1 / p47phox / p47 phox Antibody - Immunofluorescent analysis of p47 phox staining in Raji cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
NCF1 / p47phox / p47 phox Antibody - Western blot analysis of p47 phox expression in Raji (A); Ramos (B); Raw264.7 (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human NCF1 / p47phox / p47 phox Antibody (C‑Terminus, IHC, IF, WB) LS‑C368438

Polyclonal Rabbit anti‑Human NCF1 / p47phox / p47 phox Antibody (C‑Terminus, IHC, IF, WB) LS‑C368438

Antibody:
NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Bovine, Dog, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255
LS-C368438-30
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey, Mouse, Bovine, Dog, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
P47 phox antibody LS-C368438 is an unconjugated rabbit polyclonal antibody to p47 phox (NCF1 / p47phox) (C-Terminus) from human. It is reactive with human, mouse, bovine and other species. Validated for ICC, IF, IHC and WB.
Target
Human NCF1 / p47phox / p47 phox
Synonyms
NCF1 | NCF1A | Neutrophil cytosolic factor 1 | NCF-47K | Nox organizer 2 | NOXO2 | p47-phox | p47phox | SH3PXD1A | NADPH oxidase organizer 2 | NCF-1 | Neutrophil cytosol factor 1 | Nox-organizing protein 2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Bovine, Dog, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human p47 phox.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of p47 phox protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NCF1 / p47phox / p47 phox
P14598 NM_000265 NP_000256.4

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Requested From: United States
Date Requested: 11/27/2024