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Catalog Number	Size	Price
LS-C356207-100	100 µl (1 mg/ml)	$315

NCF1 / p47phox / p47 phox Antibody - Immunohistochemical analysis of p47 phox (pS359) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

NCF1 / p47phox / p47 phox Antibody - Immunofluorescent analysis of p47 phox (pS359) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

NCF1 / p47phox / p47 phox Antibody - Western blot analysis of p47 phox (pS359) expression in HeLa (A) whole cell lysates.

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Polyclonal Rabbit anti‑Human NCF1 / p47phox / p47 phox Antibody (C‑Terminus, IHC, IF, WB) LS‑C356207

Name: Anti-NCF1 / p47phox / p47 phox Antibody | Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-C356207-100
Price: 315.0000 USD
Availability: InStock

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Antibody:

NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

$315

LS-C356207-100

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

P47 phox antibody LS-C356207 is an unconjugated rabbit polyclonal antibody to human p47 phox (NCF1 / p47phox) (C-Terminus). Validated for ICC, IF, IHC and WB.

Target

Human NCF1 / p47phox / p47 phox

Synonyms

See All NCF1 / p47phox / p47 phox Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human p47 phox (pS359).

Epitope

C-Terminus

Specificity

Recognizes endogenous levels of p47 phox (pS359) protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About NCF1 / p47phox / p47 phox

Uniprot: P14598 NCBI: NM_000265 NP_000256.4