Target
Human NCAM / CD56
Synonyms
NCAM1 | CD56 | CD56 antigen | MSK39 | NCAM-1 | N-CAM-1 | NCAM
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Recombinant corresponding to soluble human CD56 (1four Ig-like domains)
Specificity
An epitope within the first four Ig-like domains of the human CD56 molecule.
Usage
Flow Cytometry: 500000 Ficoll-prepared human peripheral blood mononuclear cells were washed and pre-incubated 5 min with 20 ul of 250 ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD56 antibody at 10 ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 15% sub population of the cells stained positive with a mean shift of 0.91 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee