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Catalog Number Size Price
LS-C800677-100 100 µl (1 mg/ml) $282 
LS-C800677-200 200 µl (1 mg/ml) $350 
NBN / Nibrin Antibody
NBN / Nibrin Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
NBN / Nibrin Antibody - Western blot analysis on HuvEc cell lysates using Phospho-Nibrin(Ser278) antibody. The lane on the left is treated with the antigen-specific peptide.
NBN / Nibrin Antibody
NBN / Nibrin Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
NBN / Nibrin Antibody - Western blot analysis on HuvEc cell lysates using Phospho-Nibrin(Ser278) antibody. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human NBN / Nibrin Antibody (phospho‑Ser278, IF, WB) LS‑C800677

Polyclonal Rabbit anti‑Human NBN / Nibrin Antibody (phospho‑Ser278, IF, WB) LS‑C800677

Antibody:
NBN / Nibrin Rabbit anti-Human Polyclonal (pSer278) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$282
LS-C800677-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
NBN / Nibrin Rabbit anti-Human Polyclonal (pSer278) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Nibrin antibody LS-C800677 is an unconjugated rabbit polyclonal antibody to human Nibrin (NBN) (pSer278). Validated for IF, Peptide-ELISA and WB.
Target
Human NBN / Nibrin
Synonyms
NBN | ATV | AT-V2 | Nibrin | NBS1 | AT-V1 | NBS | p95
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Nibrin around the phosphorylation site of Sersine 278.
Epitope
pSer278
Specificity
Phospho-Nibrin(Ser278) Antibody detects endogenous levels of Nibrin only when phosphorylated at Sersine 278.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NBN / Nibrin
O60934 NM_002485 NP_002476.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 3/17/2025