Target
Human NAPSA / NAPA / Napsin A
Synonyms
NAPSA | Asp 4 | ASP4 | Aspartyl protease 4 | Kdap | NAP1 | Napsin A aspartic peptidase | Napsin-1 | KAP | TA01/TA02 | Napsin A | Napsin-A | SNAPA | NAP 1 | Pronapsin A
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clone
NAPSA/1238 + NAPSA/1239
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Recombinant fragment from the human protein.
Specificity
Human NAPSA / NAPA / Napsin A
Applications
- IHC
- IHC - Paraffin (1 - 2 µg/ml)
- Immunofluorescence (1 - 2 µg/ml)
- Western blot (1 - 2 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
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Usage
Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs. Optimal dilution of the Napsin A antibody cocktail should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Presentation
1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NAPSA / NAPA / Napsin A