Products

Research Areas

COVID-19

Resources

Distributors

Quick Order

Cart

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Cart

Products

Antibodies

All Antibodies Primary Antibodies Secondary Antibodies IHC-plus™ Antibodies PathPlus™ Antibodies Isotype Control Antibodies

ELISA and Assay Kits

All Kits Assay Kits Traditional ELISA Kits Cell-Based ELISA Kits DNA-Binding ELISA Kits Phospho-Specific ELISA Kits ELISA Development Kits Chemiluminescent CLIA Kits

Proteins

All Proteins Recombinant Proteins Native Proteins Over-Expression Lysates Bio-Active Proteins Animal-Free Proteins Synthetic Peptides Cell and Tissue Lysates

Research Areas

Cancer Research

Breast Cancer IHC Colorectal Cancer IHC Lung Cancer IHC Prostate Cancer IHC

Pathways And Groups

Apoptosis Caspases Chemokines

Infectious Disease

Virology Coronaviridae (COVID-19) Hepatitis Viruses Herpesviridae (Herpes) Orthomyxoviridae (Influenza) Papillomaviridae (HPV) Paramyxoviridae (Mumps) Pneumoviridae (RSV) Polyomaviridae (SV40) Retroviridae (HIV) Togaviridae (Measles)

Neurodegenerative Disease

Alzheimer's Disease

COVID-19

Coronavirus Detection Coronavirus and COVID-19 Inhibitors Coronavirus Antibodies, Proteins, and cDNAs Cytokine Release Syndrome General Coronavirus Information

Resources

Purchasing

Product Ordering Terms and Conditions Holiday Schedule About the LSBio Guarantee About Reviews

Reference Material

About LSBio (LifeSpan BioSciences Inc.) About Our IHC Validation Process About Our IHC-plus™ Immunohistochemistry Protocol About Our Protocols LSBio ELISA Kit Development Guide Media

Secure Logins

How To Buy

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers

Customer Service: (800) 227-6666 / (650) 697-3600

Contact Us

Additional Contact Details

Distributors

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Catalog Number	Size	Price
LS-C98243-400	400 µl	$393

NANOG Antibody - Formalin-fixed and paraffin-embedded human Spleen tissue reacted with NANOG Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

NANOG Antibody - Fluorescent confocal image of HeLa cell stained with NANOG Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NANOG primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NANOG immunoreactivity is localized to Nucleus significantly.

NANOG Antibody - Fluorescent confocal image of SY5Y cells stained NANOG antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated NANOG primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei of the SY5Y cells.

NANOG Antibody - Western blot of anti-NANOG Antibody in K562 cell line lysates (35 ug/lane). NANOG (arrow) was detected using the purified antibody.Western blot of NANOG (arrow) using rabbit polyclonal NANOG Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).

NANOG Antibody - NANOG Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

1 of 5

2 of 5

3 of 5

4 of 5

5 of 5

Polyclonal Rabbit anti‑Human NANOG Antibody (aa15‑49, IHC, IF, WB) LS‑C98243

Name: Anti-NANOG Antibody | Rabbit anti-Human Polyclonal Flo,IF,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C98243-400
Price: 393.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

NANOG Rabbit anti-Human Polyclonal (aa15-49) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

$393

LS-C98243-400

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

NANOG Rabbit anti-Human Polyclonal (aa15-49) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

NANOG antibody LS-C98243 is an unconjugated rabbit polyclonal antibody to human NANOG (aa15-49). Validated for Flow, IF, IHC and WB.

Target

Human NANOG

Synonyms

NANOG | Nanog homeobox | NANOGP8 | HNanog | Homeobox protein NANOG

See All NANOG Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Predicted

Monkey (at least 90% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Ammonium sulfate precipitation

Modifications

Unmodified

Epitope

aa15-49

Specificity

This NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-49 amino acids from the N-terminal region of human NANOG.

Applications

IHC
IHC - Paraffin (1:10 - 1:50)
Immunofluorescence (1:10 - 1:50)
Western blot (1:1000)
Flow Cytometry (1:10 - 1:50)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Failed Applications