Human Myosin

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Antiserum

Unmodified

Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH).

pSer19/20

This antiserum is phosphospecific and detects monophosphorylated and diphosphorylated forms of the protein. Reactivity with non-phosphorylated myosin light chain is less than 1% by ELISA. Cross reactivity is expected with myosin light chain from human and mouse. Reactivity with the protein from other species has not been determined. However, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.

• IHC (2.5 µg/ml)
• Western blot (1:1000 - 1:5000)
• Immunoprecipitation (1:100)
• ELISA (1:5000 - 1:20000)

Applications should be user optimized.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.