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Catalog Number	Size	Price
LS-C312448-10	10 µg	$318
LS-C312448-100	100 µg (0.1 mg/ml)	$470

MYH7 Antibody - Western blot analysis of Myosin(Skeletal, Slow) using anti-Myosin(Skeletal, Slow) antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse skeletal muscle tissue lysates, Lane 2: rat skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Myosin(Skeletal, Slow) antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Myosin(Skeletal, Slow) at approximately 200-220KD. The expected band size for Myosin(Skeletal, Slow) is at 220KD.

MYH7 Antibody - WB of MYH7 antibody. All lanes: Anti-Myosin(slow) at 0.5ug/ml. Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug. Lane 2: Mouse Skeletal Muscle Tissue Lysate at 40ug. Predicted bind size: 200KD. Observed bind size: 200KD.

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Monoclonal Mouse anti‑Human MYH7 Antibody (clone IML‑64, IHC, WB) LS‑C312448

Name: Anti-MYH7 Antibody | Mouse anti-Human Monoclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C312448-10
Price: 318.0000 USD
Availability: InStock
Rating: 4.1 (1 reviews)

(4.1) Rating

Submit Review (2) Publication(s) LSBio Guarantee

Antibody:

MYH7 Mouse anti-Human Monoclonal (IML-64) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Mouse, Rat, Rabbit

Format:

Unconjugated, Unmodified

$318

LS-C312448-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

MYH7 Mouse anti-Human Monoclonal (IML-64) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Mouse, Rat, Rabbit

Format:

Unconjugated, Unmodified

Specifications

Description

MYH7 antibody LS-C312448 is an unconjugated mouse monoclonal antibody to MYH7 from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Cited in 2 publications.

(2) Publication(s)

Target

Human MYH7

Synonyms

See All MYH7 Antibodies

Host

Mouse

Reactivity

Human, Mouse, Rat, Rabbit (tested or 100% immunogen sequence identity)

Clonality

IgG1 Monoclonal

Clone

IML-64

Conjugations

Unconjugated

Purification

Ascites

Modifications

Unmodified

Immunogen

Human skeletal muscle myosin purified from myofibrils.

Specificity

MYH7: Both wild type and variant Gln-403 aredetected in skeletal muscle (at protein level).

Applications

IHC
IHC - Paraffin
Western blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from ascites, 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide.

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About MYH7

Uniprot: P12883 NCBI: M58018 P12883

LSBio Ratings

MYH7 Antibody (clone IML-64) for IHC, WB/Western LS-C312448 has an LSBio Rating of

(4.1) Rating

Publications (4.1)

Learn more about The LSBio Ratings Algorithm

Publications (2)

Characterization of cytoskeleton features and maturation status of cultured human iPSC-derived cardiomyocytes. Christian Zuppinger, George Gibbons, Priyanka Dutta-Passecker, Adrian Segiser, Henriette Most, Thomas M Suter. European journal of histochemistry : EJH. 2017 Jun;61:2763.

Full Text Article PubMed: 28735524 PMC: PMC5484009

3D Co-culture of hiPSC-Derived Cardiomyocytes With Cardiac Fibroblasts Improves Tissue-Like Features of Cardiac Spheroids. Philippe Beauchamp, Christopher B Jackson, Lijo Cherian Ozhathil, Irina Agarkova, Cristi L Galindo, Douglas B Sawyer, Thomas M Suter, Christian Zuppinger. Frontiers in molecular biosciences. 2020 Feb;7:14.

Full Text Article PubMed: 32118040 PMC: PMC7033479

Submit a Publication for LS-C312448