Target
Human MTBP / Mdm2-Binding Protein
Synonyms
MTBP | Mdm2-binding protein | MDM2BP | HMTBP
Reactivity
Human, Chimpanzee
(tested or 100% immunogen sequence identity)
Predicted
Chimpanzee (at least 90% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant amino-terminal HIS6X-tagged polypeptide corresponding to amino acid residues 667-812 of human MTBP protein.
Specificity
This antibody is directed against human MTBP protein. The product was produced from clarified mouse ascetic fluid. A BLAST analysis was used to suggest reactivity with this protein from human (100% homology) and chimpanzee. Cross reactivity with MTBP protein from mouse (77% homology) and chicken (79% homology) is unlikely due to the relatively low number of positive residues and high number of gaps within this sequence. Cross reactivity with MTBP homologues from other sources has not been determined.
Applications
- Western blot (1:200 - 1:2000)
- ELISA (1:2000 - 1:20000)
Usage
This antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band - 110 kD in size corresponding to MTBP by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MTBP / Mdm2-Binding Protein