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Antibodies > MSH2 > LS-C357472
 
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Catalog Number Size Price
LS-C357472-10 10 µg $318 
LS-C357472-100 100 µg (0.5 mg/ml) $470 
MSH2 Antibody - MSH2 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
MSH2 Antibody - MSH2 antibody IHC-paraffin: Rat Intestine Tissue.
MSH2 Antibody - MSH2 antibody IHC-paraffin: Mouse Intestine Tissue.
MSH2 Antibody - MSH2 antibody ICC: SMMC Cell.
MSH2 Antibody - IF analysis of MSH2 using anti-MSH2 antibody MSH2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-MSH2 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MSH2 Antibody - Western blot analysis of MSH2 using anti-MSH2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human COLO-320 whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSH2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MSH2 at approximately 105KD. The expected band size for MSH2 is at 105KD.
MSH2 Antibody - MSH2 antibody Western blot. All lanes: Anti MSH2 at 0.5 ug/ml. Lane 1: Mouse Testis Tissue Lysate at 50 ug. Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Lane 4: A549 Whole Cell Lysate at 40 ug. Lane 5: SMMC Whole Cell Lysate at 40 ug. Predicted band size: 105 kD. Observed band size: 105 kD.
MSH2 Antibody - Flow Cytometry analysis of A431 cells using anti-MSH2 antibody. Overlay histogram showing A431 cells stained with anti-MSH2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSH2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
MSH2 Antibody - MSH2 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
MSH2 Antibody - MSH2 antibody IHC-paraffin: Rat Intestine Tissue.
MSH2 Antibody - MSH2 antibody IHC-paraffin: Mouse Intestine Tissue.
MSH2 Antibody - MSH2 antibody ICC: SMMC Cell.
MSH2 Antibody - IF analysis of MSH2 using anti-MSH2 antibody MSH2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-MSH2 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MSH2 Antibody - Western blot analysis of MSH2 using anti-MSH2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human COLO-320 whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSH2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MSH2 at approximately 105KD. The expected band size for MSH2 is at 105KD.
MSH2 Antibody - MSH2 antibody Western blot. All lanes: Anti MSH2 at 0.5 ug/ml. Lane 1: Mouse Testis Tissue Lysate at 50 ug. Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Lane 4: A549 Whole Cell Lysate at 40 ug. Lane 5: SMMC Whole Cell Lysate at 40 ug. Predicted band size: 105 kD. Observed band size: 105 kD.
MSH2 Antibody - Flow Cytometry analysis of A431 cells using anti-MSH2 antibody. Overlay histogram showing A431 cells stained with anti-MSH2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSH2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human MSH2 Antibody (aa337‑583, IHC, WB) LS‑C357472

Polyclonal Rabbit anti‑Human MSH2 Antibody (aa337‑583, IHC, WB) LS‑C357472

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Note: This antibody replaces LS-C388898
Antibody:
MSH2 Rabbit anti-Human Polyclonal (aa337-583) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C357472-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MSH2 Rabbit anti-Human Polyclonal (aa337-583) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
MSH2 antibody LS-C357472 is an unconjugated rabbit polyclonal antibody to MSH2 (aa337-583) from human. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB.
Target
Human MSH2
Synonyms
MSH2 | COCA1 | FCC1 | HNPCC | HNPCC1 | MutS protein homolog 2 | HMSH2 | LCFS2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human MSH2 recombinant protein (Position: Q337-N583). Human MSH2 shares 94% and 93% amino acid (aa) sequence identity with mouse and rat MSH2, respectively.
Epitope
aa337-583
Specificity
Ubiquitously expressed.
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MSH2
P43246 NM_000251 NP_000242.1

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MSH2 Antibody - Immunohistochemical analysis of MSH2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MSH2 Antibody (Internal) LS‑C368893
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Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
MSH2 Antibody - Anti-MSH2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Applications: IHC - Paraffin, IHC - Frozen
MSH2 ELISA Kit
Human MSH2 (Sandwich ELISA) ELISA Kit - LS-F35503
Reactivity: Human
Range: 78-5000 pg/ml
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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United States

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