Human IgM

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

NI 179

Horseradish Peroxidase. Also available Unconjugated or conjugated with FITC, TRITC, Biotin.

Purified IgG

Unmodified

Highly purified monoclonal IgM isolated from pooled human serum.

Human IgM, subclass specific.

• IHC
• IHC - Paraffin
• ICC
• ELISA
• Dot Blot
• Applications tested for the base form of this product only

The reactivity of the antiserum is restricted to an isotype specific determinant of the IgM molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgM, and immunoblotting. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200. in ELISA from 1:250 upwards. in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from PBS, pH 7.2, No preservatives added

Reconstitute with distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.