Human IgM

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

MuHC2

Unconjugated

Protein G purified

Unmodified. Also available Ready-to-Use, Azide-free, BSA-free.

Human Mu heavy chain.

Human IgM

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (0.5 - 1 µg/ml)
• Western blot (0.5 - 1 µg/ml)

IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen binding sites (due to the large size of most antigens, not all binding sites can be filled simultaneously. IgM antibodies account for approximately 5%-10% of all the antibody in the body. Due to differences in protocols and secondaries, the IgM antibody may need to be titered for optimal performance. FFPE staining requires boiling sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS, pH 7.4, 0.1 mg/ml BSA, 0.05% Sodium Azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.