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Antibodies > MMP8 > LS-C352524
 
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Catalog Number Size Price
LS-C352524-100 100 µl (1 mg/ml) $299 
MMP8 Antibody - Immunohistochemical analysis of MMP8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MMP8 Antibody - Immunofluorescent analysis of MMP8 staining in SW620 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MMP8 Antibody - Western blot analysis of MMP8 expression in SJRH30 (A); PMNL (B); Sw620 (C) whole cell lysates.
MMP8 Antibody - Immunohistochemical analysis of MMP8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MMP8 Antibody - Immunofluorescent analysis of MMP8 staining in SW620 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MMP8 Antibody - Western blot analysis of MMP8 expression in SJRH30 (A); PMNL (B); Sw620 (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human MMP8 Antibody (C‑Terminus, IHC, IF, WB) LS‑C352524

Polyclonal Rabbit anti‑Human MMP8 Antibody (C‑Terminus, IHC, IF, WB) LS‑C352524

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Antibody:
MMP8 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352524-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MMP8 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
MMP8 antibody LS-C352524 is an unconjugated rabbit polyclonal antibody to MMP8 (C-Terminus) from human. It is reactive with human and rat. Validated for ICC, IF, IHC and WB.
Target
Human MMP8
Synonyms
MMP8 | CLG1 | HNC | Matrix metalloproteinase-8 | MMP-8 | Neutrophil collagenase | PMNL collagenase | PMNL-CL
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human MMP8.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of MMP8 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MMP8
P22894 NM_002424 NP_002415.1

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MMP8 Antibody - Staining LOVO cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
MMP8 Antibody LS‑C801517
Species: Human, Mouse, Rat
Applications: IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Flow Cytometry, Peptide Enzyme-Linked Immunosorbent Assay
MMP8 Antibody - Western Blot; Sample: Rat Liver lysate; Primary Ab: 2µg/ml Rabbit Anti-Rat MMP8 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
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Species: Rat, Mouse
Applications: Western blot
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Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
See all MMP8 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/10/2025
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