Human MMP3

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated

Protein A purified

Unmodified

APMA (4-Aminophenylmercuric acetate) activated Human stromelysin-1.

Recognizes one doublet of 54kD/59kD and another doublet of 44kD/49kD, which are identified as unglycosylated and glycosylated species of pro (latent) and active forms of matrix metalloproteinase-3 (MMP-3; also known as Stromelysin-1 of Transin). No cross-reactivity with the pro and active forms of other MMPs. Recognizes human.

• IHC
• IHC - Paraffin
• IHC - Frozen
• Immunofluorescence
• Western blot (0.5 - 1 µg/ml)
• Immunoprecipitation (2 µg/ml)

Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Use Protein A): 2 ug/ml Protein lysate. Western Blot: 0.5-1.0 ug/ml for 2hrs at RT. Immunohistochemistry (frozen & formalin-fixed):10-20g/ml for 60 minute at RT. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues. Positive control: Human endometrium cells, placenta, bladder, breast, ovarian carcinomas.

PBS, pH 7.4, 0.2% BA, 0.09% sodium azide.

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08254 NCBI: NM_002422 NP_002413.1