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Catalog Number Size Price
LS-C352523-100 100 µl (1 mg/ml) $299 
MMP2 Antibody - Immunohistochemical analysis of MMP2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MMP2 Antibody - Immunofluorescent analysis of MMP2 staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MMP2 Antibody - Western blot analysis of MMP2 expression in HUVEC (A); NRK (B); MCF7 (C); HT1080 (D) whole cell lysates.
MMP2 Antibody - Immunohistochemical analysis of MMP2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MMP2 Antibody - Immunofluorescent analysis of MMP2 staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MMP2 Antibody - Western blot analysis of MMP2 expression in HUVEC (A); NRK (B); MCF7 (C); HT1080 (D) whole cell lysates.
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Polyclonal Rabbit anti‑Human MMP2 Antibody (C‑Terminus, IHC, IF, WB) LS‑C352523

Polyclonal Rabbit anti‑Human MMP2 Antibody (C‑Terminus, IHC, IF, WB) LS‑C352523

Antibody:
MMP2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352523-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
MMP2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
MMP2 antibody LS-C352523 is an unconjugated rabbit polyclonal antibody to MMP2 (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human MMP2
Synonyms
MMP2 | 72 kd type iv collagenase | 72 kDa gelatinase | 72 kd gelatinase | 72 kDa type IV collagenase | CLG4A | CLG4 | GelA | Gelatinase A | Matrix metalloproteinase-2 | MMP-2 | MONA | Neutrophil gelatinase | TBE-1 | Tbe-1 metalloprotease | Matrix metalloproteinase-II | MMP-II
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human MMP2.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of MMP2 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MMP2
P08253 NM_004530 NP_004521.1

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Requested From: United States
Date Requested: 11/24/2024