Target
Human MLC2 / MYL9
Synonyms
MYL9 | 20 kDa myosin light chain | LC20 | MLC-2C | MLC2 | Myosin RLC | MRLC1 | MYRL2
Reactivity
Human, Mouse, Rat, Bovine, Pig
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Immunogen
Phospho-peptide corresponding to residues surroundingSer19 of human myosin light chain 2 (smooth muscle) (KLH coupled).
Specificity
Detects endogenous levels of human myosin light chain 2 (smooth muscle) only when phosphorylated at serine 19 at 18kD. Does not cross-react with the cardiac isoform of myosin light chain 2. Species cross-reactivity: mouse and rat.
Applications
- Immunofluorescence (1:200)
- Western blot (1:1000)
Usage
Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Immunocytochemistry. Western Blot: 1:1000. Immunohistochemistry: 1:100. Immunocytochemistry: (IF) 1:50.
Presentation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1mg/ml BSA, <0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee