Human MKI67 / Ki67

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Ki-S5

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Nuclear protein preparation from the human cell line U937.50.

Recognizes a cell-cycle-associated protein of 345kD and 395kD identical with the human Ki-67 antigen. Binds to a formalin-resistant epitope of the Ki-67 antigen. Confined to the nuclei of proliferating cells and no cross-reactivity with cytoplasmic antigens of epithelial occurs even after antigen retrieval. Immunohistochemistry labeling of fresh and frozen samples showed identical results with Ki-67 and Ki-S5.

• IHC
• IHC - Paraffin (10 µg/ml)
• ICC (5 - 10 µg/ml)
• Western blot (1:500)
• Flow Cytometry

Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Western Blot: 1:500. Detected cell-cycle-associated protein of 345kD and 395kD on 10 ug of A431 lysates. Immunocytochemistry: 5-10 ug/ml. Immunohistochemistry: 5-10 ug/ml. Positive control: Tonsil tissue, A431 Cell Lysate.

PBS, pH 7.6, 0.09% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P46013 NCBI: NM_002417 NP_002408.3