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| Catalog Number | Size | Price |
|---|---|---|
| LS-B6433-50 | 50 µg (1 mg/ml) | $485 |
IHC‑plus™ Monoclonal Mouse anti‑Human MKI67 / Ki67 Antibody (clone Ki‑S5, IHC, WB) LS‑B6433
IHC‑plus™ Monoclonal Mouse anti‑Human MKI67 / Ki67 Antibody (clone Ki‑S5, IHC, WB) LS‑B6433
Note: This antibody replaces LS-C25007
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
Ki67 antibody LS-B6433 is an unconjugated mouse monoclonal antibody to human Ki67 (MKI67). Validated for Flow, ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human MKI67 / Ki67
Synonyms
MKI67 | Antigen KI-67 | Ki67 | KIA
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
Ki-S5
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Nuclear protein preparation from the human cell line U937.50.
Specificity
Recognizes a cell-cycle-associated protein of 345kD and 395kD identical with the human Ki-67 antigen. Binds to a formalin-resistant epitope of the Ki-67 antigen. Confined to the nuclei of proliferating cells and no cross-reactivity with cytoplasmic antigens of epithelial occurs even after antigen retrieval. Immunohistochemistry labeling of fresh and frozen samples showed identical results with Ki-67 and Ki-S5.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- ICC (5 - 10 µg/ml)
- Western blot (1:500)
- Flow Cytometry
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Western Blot: 1:500. Detected cell-cycle-associated protein of 345kD and 395kD on 10 ug of A431 lysates. Immunocytochemistry: 5-10 ug/ml. Immunohistochemistry: 5-10 ug/ml. Positive control: Tonsil tissue, A431 Cell Lysate.
Presentation
PBS, pH 7.6, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MKI67 / Ki67
Validation
Anti-MKI67 / Ki-67 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-MKI67 / Ki-67 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ MKI67 / Ki67 Antibody (clone Ki-S5) for IHC, ICC, WB/Western, Flow LS-B6433 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Targeting stanniocalcin-1-expressing tumor cells elicits efficient antitumor effects in a mouse model of human lung cancer. Kotaro Abe, Masahiko Kanehira , Shinya Ohkouchi, Sakiko Kumata, Yamato Suzuki, Hisashi Oishi, Masafumi Noda, Akira Sakurada, Eisaku Miyauchi, Tohru Fujiwara, Hideo Harigae, Yoshinori Okada. Cancer medicine. 2021 May;;10:3085-3100.
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Featured Products
Species:
Mouse, Human, Rat
Applications:
IHC, IHC - Paraffin, ICC, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence
![MKI67 / Ki67 Antibody - Immunofluorescence of monoclonal antibody to MKI67 on HeLa cell . [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/mki67-ki67-antibody-clone-7b8-ls-c197685/145664_904180.jpg)
Species:
Human
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Mouse, Human
Applications:
IHC, IHC - Paraffin, ICC
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
Date Requested: 12/22/2024
