Target
Mouse MIP2 / GRO2 / CXCL2
Synonyms
CXCL2 | C-X-C motif chemokine 2 | CINC-2a | GROb | Growth-regulated protein beta | MIP-2a | MIP2-alpha | MGSA-b | MIP-2 | SCYB2 | MGSA beta | MIP2 | Gro-beta | GRO2 | GRO2 oncogene | MIP2A
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Biotin.
Also available
Unconjugated.
Purification
Immunoaffinity purified
Immunogen
E.coli derived recombinant Murine MIP-2.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- ELISA (0.25 - 1 µg/ml)
- Applications tested for the base form of this product only
Usage
ELISA: To detect mMIP-2 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mMIP-2. To detect mMIP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine MIP-2 as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mMIP-2. Western Blot: To detect mMIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile PBS containing 0.1% BSA
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MIP2 / GRO2 / CXCL2