Target
Human MECOM / EVI1
Synonyms
MECOM | AML1-EVI-1 | EVI-1 | EVI1 | MDS1 | MDS1 and EVI1 complex locus | MDS1-EVI1 | Oncogene EVI1 | AML1-EVI-1 fusion protein | Zinc finger protein Evi1 | Myelodysplasia syndrome 1 | PRDM3
Reactivity
Human, Monkey, Dog, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-DKESLHSTSH, from the internal region of the protein sequence according to NP_001098547.3NP_005232.2NP_004982.2NP_001157471.1NP_001157472.1.
Specificity
Human MECOM. This antibody is expected to recognize all reported isoforms (NP_001098547.3; NP_005232.2; NP_004982.2; NP_001157471.1; NP_001157472.1). Reported variants represent identical protein: NP_001098548.2, NP_005232.2
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 170kDa band observed in lysates of cell lines A549 and HepG2 (calculated MW of 126kDa according to NP_001098547.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee