Target
Human MDFI / I-MF
Synonyms
MDFI | I-MF | I-mfa | MyoD family inhibitor | Inhibitor of MyoD family a | Myogenic repressor I-mf
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence YQVSGQRPSGCD, from the N-Terminus of protein sequence according to NP_005577.1.
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Preliminary experiments gave an approx 20kDa band in Human Skeletal Muscle lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25.0kDa according to NP_005577.1. The 20kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee