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Catalog Number Size Price
LS-C287647-10 10 µl $295 
LS-C287647-100 100 µl (0.2 mg/ml) $533 
MCM2 Antibody - Detection of Human Phospho MCM2 (S40/S41) by Immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) ( Lot 2) used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
MCM2 Antibody - Detection of Human Phospho MCM2 (S40/S41) by Western Blot. Samples: Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S40/S41) antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).
MCM2 Antibody - Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 X 10e6 cells were stained with 0.1 ug/ml anti-phospho-MCM2 (pS40/41) [] in a 150 mcl volume. DNA content was simultaneously analyzed via PI stain.
MCM2 Antibody - Detection of Human Phospho MCM2 (S40/S41) by Immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) ( Lot 2) used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
MCM2 Antibody - Detection of Human Phospho MCM2 (S40/S41) by Western Blot. Samples: Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S40/S41) antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).
MCM2 Antibody - Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 X 10e6 cells were stained with 0.1 ug/ml anti-phospho-MCM2 (pS40/41) [] in a 150 mcl volume. DNA content was simultaneously analyzed via PI stain.
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Polyclonal Rabbit anti‑Human MCM2 Antibody (phospho‑Ser40/41, IHC, WB) LS‑C287647

Polyclonal Rabbit anti‑Human MCM2 Antibody (phospho‑Ser40/41, IHC, WB) LS‑C287647

Antibody:
MCM2 Rabbit anti-Human Polyclonal (pSer40/41) Antibody
Application:
IHC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C287647-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
MCM2 Rabbit anti-Human Polyclonal (pSer40/41) Antibody
Application:
IHC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MCM2 antibody LS-C287647 is an unconjugated rabbit polyclonal antibody to human MCM2 (pSer40/41). Validated for Flow, IHC and WB.
Target
Human MCM2
Synonyms
MCM2 | BM28 | Cell devision cycle-like 1 | D3S3194 | Cdc19 | Cyclin-like 1 | KIAA0030 | Nuclear protein BM28 | CDCL1 | MITOTIN
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 4171 corresponding to amino acids surrounding serine 40/41 of UniprotID: P49736
Epitope
pSer40/41
Specificity
Human MCM2 (phospho-Ser40)
Applications
  • IHC (1:100 - 1:500)
  • Western blot (1:1000 - 1:5000)
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Flo: 0.1 µg per 1 X 10^6 cells in a 150 µl volume
Manufacturer
Bethyl Laboratories, Inc.
Presentation
TBS, 0.09% Sodium Azide, 0.1% BSA
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCM2
P49736 NM_004526 NP_004517.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/22/2024