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Antibodies > mCherry > LS-C204826
 
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Catalog Number Size Price
LS-C204826-100 100 µl (1 mg/ml) $376 
mCherry Antibody - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome. Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.
mCherry Antibody - Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, in the lane marked plus. HEK293 cells which were not transfected with this vector show no protein band in lane marked minus.
mCherry Antibody - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome. Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.
mCherry Antibody - Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, in the lane marked plus. HEK293 cells which were not transfected with this vector show no protein band in lane marked minus.
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Polyclonal Rabbit anti‑Discosoma mCherry Antibody (IF, WB) LS‑C204826

Polyclonal Rabbit anti‑Discosoma mCherry Antibody (IF, WB) LS‑C204826

(4.5) Rating
(1) Review(s) (1) Publication(s) LSBio Guarantee
Antibody:
mCherry Rabbit anti-Discosoma Polyclonal Antibody
Application:
ICC, IF, WB
Format:
Unconjugated, Unmodified
Price
Catalog Number
$376
LS-C204826-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
mCherry Rabbit anti-Discosoma Polyclonal Antibody
Application:
ICC, IF, WB
Format:
Unconjugated, Unmodified

Specifications

Description
MCherry antibody LS-C204826 is an unconjugated rabbit polyclonal antibody to mCherry from discosoma. Validated for ICC, IF and WB. Cited in 1 publication.
(1) Publication(s)
Target
Discosoma mCherry
Host
Rabbit
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant full length His tagged mCherry purified from E. coli.
Specificity
mCherry
Applications
  • ICC (1:500)
  • Immunofluorescence (1:500)
  • Western blot (1:1000)
Usage
Try at dilutions of 1:500 and higher for immunofluorescence. For western blots try at 1:1000. The mCherry protein runs at about 30kDa on SDS-PAGE gels.
Presentation
10 mM Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

LSBio Ratings

mCherry Antibody for ICC, IF/Immunofluorescence, WB/Western LS-C204826 has an LSBio Rating of
(4.5) Rating
Publications (4)
Customer Reviews (5)

Publications (1)

PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading. Rona G, Roberti D, Yin Y, Pagan JK, Homer H, Sassani E, Zeke A, Busino L, Rothenberg E, Pagano M. eLife. 2018 July;7:pii: e38771. (Discosoma)
Full Text Article PubMed: 29985131 PMC: PMC6037479
Submit a Publication for LS-C204826

Customer Reviews (1)

Rating
(5)
Reviewed By
Anonymous LSBio Customer
Submitted
December 16, 2016
Applications
IHC-PFA
Species
Mouse
Details
Application: Immunohistochemistry (PFA fixed frozen sections)
Sample Type: Frozen mouse kidney
Sample Fixative: 4% PFA
Antigen Retrieval: None
Permeabilization: None
Blocking: PBS + NDS 2% + Triton-X100 0.1%
Primary Ab Dilution: 1:500
Primary Ab Incubation Buffer: PBS + NDS 1% + Triton-X100 0.1%
Primary Ab Incubation Time: Overnight
Primary Ab Incubation Temp: 4°C
Secondary Ab Name: Donkey anti-Rabbit, Alexa Fluor 594
Secondary Ab Host: Donkey
Secondary Ab Clonality: Polyclonal
Secondary Ab Conjugation: Alexa Fluor 594
Secondary Dilution: 1:500
Detection Method: Confocal microscopy
Submit a review for LS-C204826

Featured Products

mCherry Antibody - 293 HEK cells transduced with Ad mCherry Rab1a and stained with anti-mCherry
mCherry Antibody LS‑C204207
Species: Discosoma
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot
mCherry Antibody - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger Cherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight Cherry fluorochrome.
mCherry Antibody LS‑C204825
Species: Discosoma
Applications: ICC, Immunofluorescence, Western blot
mCherry Antibody - Shows HEK293 cells transfected with mCherry and visualized in red. The cells were stained with mCherry antibody in the green channel, and visualized using a confocal microscope. Transfected cells are yellow, showing overlap of the mCherry and the mCherry antibody antibody. Untransfected HEK293 cells do not express Cherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue). Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.
mCherry Antibody (clone 1C51) LS‑C204827
Species: Discosoma
Applications: ICC, Immunofluorescence, Western blot
mCherry Antibody - HEK293T cells were transfected with the pCMV6-ENTRY mCherry cDNA and the cell lysates were collected after 48 hours. 0ug, 0.1ug, 0.2ug, 0.4ug, 0.8ug, 1.6ug of lysates. (lane 1 to lane 6 respectively) were separated by SDS-PAGE and immunoblotted with Rabbit mCherry polyclonal antibody at 1:10000.
mCherry Antibody LS‑C784928
Species:
Applications: Western blot
See all mCherry Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
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