Target
Human MAPK8 / JNK1
Synonyms
MAPK8 | JNK | JNK1 | JNK1A2 | JNK-46 | MAP kinase 8 | PRKM8 | SAPK1 | SAPK1c | MAPK 8 | C-Jun N-terminal kinase 1 | JNK21B1/2 | JUN N-terminal kinase
Reactivity
Human, Mouse, Rat, Chicken, Drosophila, C. elegans
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein A purified
Immunogen
Synthetic peptide corresponding to aa181-190 [(MM[pT]P[pY]VVTRY-C)] of human/mouse/rat JNK1 (MAPK 8) and human/mouse/rat JNK2 (MAPK 9). Sequence also matches aa219-229 of human/mouse/rat JNK3.
Epitope
pThr183/Tyr185/Thr221/Tyr223
Specificity
Recognizes phosphorylated JNK, Mr 46kD and 55kD. Species cross-reactivity: Human, rat, mouse. Expected to cross-react with Drosophila, C. elegans and chicken based on sequence homology.
Applications
- Western blot (1 µg/ml)
- Immunoprecipitation (1 µg/ml)
Usage
Suitable for use in Immunoprecipitation/Western Blot. Immunoprecipitation/Western Blot: 1 ug/ml. Anti-pan JNK/SAPK1 was used to Immunoprecipitate JNK from lysates of ultraviolet light-treated RAW 264 macrophage. Phosphorylated JNK (T183/Y185) was detected. Phosphorylated JNK was not detected in non-treated lysates.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee