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Catalog Number	Size	Price
LS-C15060-200	200 µl	$854

Polyclonal Sheep anti‑Human MAPK7 / ERK5 Antibody (phospho‑Thr218/Tyr220, WB) LS‑C15060

Name: Anti-MAPK7 / ERK5 Antibody | Sheep anti-Human Polyclonal WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C15060-200
Price: 854.0000 USD
Availability: InStock

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Antibody:

MAPK7 / ERK5 Sheep anti-Human Polyclonal (pThr218/Tyr220) Antibody

Application:

Reactivity:

Human

Format:

Unconjugated, Unmodified

Other formats:

APC Biotin FITC HRP PE

$854

LS-C15060-200

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

MAPK7 / ERK5 Sheep anti-Human Polyclonal (pThr218/Tyr220) Antibody

Application:

Reactivity:

Human

Format:

Unconjugated, Unmodified

Other formats:

APC Biotin FITC HRP PE

Specifications

Description

ERK5 antibody LS-C15060 is an unconjugated sheep polyclonal antibody to human ERK5 (MAPK7) (pThr218/Tyr220). Validated for WB.

Target

Human MAPK7 / ERK5

Synonyms

MAPK7 | Big MAP kinase 1 | BMK-1 | Erk4 | ERK5 | MAPK 7 | PRKM7 | BMK1 | BMK1 kinase | ERK-5 | MAP kinase 7

See All MAPK7 / ERK5 Antibodies

Host

Sheep

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

Synthetic peptide corresponding to amino acids 213-224 of human BMK1/Erk5 (HQYFM[pT]E[pY]VATR), KLH-conjugated. The immunizing sequence is identical in mouse, 9/12aa identical in rat.

Epitope

pThr218/Tyr220

Specificity

Recognizes human phospho-BMK1/Erk5 (Thr218, Tyr220), Mr ~110kD. Predicted to cross-react with mouse and rat based on sequence homology.

Applications

Western blot

Failed Applications

IHC - Paraffin

Usage

Suitable for use in Western Blot. Western Blot: 1-:250-1:500 detects phospho-BMK1/Erk5 (Thr218, Tyr220) in RIPA lysates from HeLa cells treated with sorbitol. Lysates from untreated or sorbitol-treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with either anti-phospho-BMK1/Erk5 (Thr218, Tyr220) (1:500) or anti-BMK1/Erk5 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (BMK1/Erk5 or phospho- BMK1/Erk5 (110kD).

Presentation

100 mM Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About MAPK7 / ERK5

Uniprot: Q13164 NCBI: NM_002749 NP_002740.2