Human MAP3K5 / ASK1

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Unmodified

This purified antibody was prepared from rabbit serum after repeated immunizations with a KLH conjugated peptide corresponding to an internal region near amino acids 75-100 of human ASK-1 protein.

pSer83

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed with ASK-1 from mouse sources. Reactivity with the kinase from other sources has not been determined.

• Western blot (1:1000)
• ELISA (1:5000 - 1:10000)

This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimal reactivity by western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, immunohistochemistry and immunoprecipitation. A 155 kD band corresponding to human ASK-1 is detected. Whole cell lysates from SW1353 can be used as a positive control.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q99683 NCBI: NM_005923 NP_005914.1