Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C352843-100 100 µl (1 mg/ml) $315 
MAP2K4 / MKK4 Antibody - Immunohistochemical analysis of MKK4 (pS80) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MAP2K4 / MKK4 Antibody - Immunofluorescent analysis of MKK4 (pS80) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MAP2K4 / MKK4 Antibody - Western blot analysis of MKK4 (pS80) expression in HEK293 UV-treated (A) whole cell lysates.
MAP2K4 / MKK4 Antibody - Immunohistochemical analysis of MKK4 (pS80) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
MAP2K4 / MKK4 Antibody - Immunofluorescent analysis of MKK4 (pS80) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MAP2K4 / MKK4 Antibody - Western blot analysis of MKK4 (pS80) expression in HEK293 UV-treated (A) whole cell lysates.
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human MAP2K4 / MKK4 Antibody (phospho‑Ser80, IHC, IF, WB) LS‑C352843

Polyclonal Rabbit anti‑Human MAP2K4 / MKK4 Antibody (phospho‑Ser80, IHC, IF, WB) LS‑C352843

Antibody:
MAP2K4 / MKK4 Rabbit anti-Human Polyclonal (pSer80) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$315
LS-C352843-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MAP2K4 / MKK4 Rabbit anti-Human Polyclonal (pSer80) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
MKK4 antibody LS-C352843 is an unconjugated rabbit polyclonal antibody to MKK4 (MAP2K4) (pSer80) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB.
Target
Human MAP2K4 / MKK4
Synonyms
MAP2K4 | JNK activating kinase 1 | JNK-activated kinase 1 | JNK-activating kinase 1 | MAPK/ERK kinase 4 | MAPKK4 | JNKK1 | PRKMK4 | SAPKK1 | SEK1 | MEK4 | MKK4 | SAPK/ERK kinase 1 | SERK1 | JNKK | MAP kinase kinase 4 | MAPKK 4 | MEK 4 | SAPK kinase 1 | SAPKK-1 | SKK1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human MKK4.
Epitope
pSer80
Specificity
Recognizes endogenous levels of MKK4 (pS80) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAP2K4 / MKK4
P45985 NM_003010 NP_003001.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024