Target
Bovine LPO / Lactoperoxidase
Synonyms
LPO | Lactoperoxidase | Salivary peroxidase | SPO | SAPX
Reactivity
Bovine
(tested or 100% immunogen sequence identity)
Purification
Purified IgG
Immunogen
Lactoperoxidase (EC 1.11.1.7) is one of the major enzymes in bovine milk. The enzyme has a molecular weight of 80000. It sis isolated from pooled bovine milk. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Bovine lactoperoxidase.
Applications
- IHC
- IHC - Paraffin
- ICC
- ELISA
- Dot Blot
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Usage
The reactivity of the antiserum is restricted to lactoperoxidase as tested in immunoelectrophoresis and radial immunodiffusion. Using various antiserum concentrations against fresh bovine milk a single precipitin line is obtained. No precipitation reaction is obtained with normal bovine serum or plasma. In immunocytochemical and immunohistochemical staining to identify and measure lactoperoxidase at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. Working dilutions for histochemical use are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:5000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LPO / Lactoperoxidase