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Antibodies > LL37 / Cathelicidin > LS-C93988
 
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Catalog Number Size Price
LS-C93988-500 500 µg $393 
LL37 / Cathelicidin Antibody - Sheep antibody to LL-37. Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffy coat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Sheep antibody to human Cathelicidin antimicrobial peptide: IgG diluted 50 ug/ml: in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI. The antibody selectively recognizes polymorphonuclear cells.
LL37 / Cathelicidin Antibody - Sheep antibody to LL-37. Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffy coat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Sheep antibody to human Cathelicidin antimicrobial peptide: IgG diluted 50 ug/ml: in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI. The antibody selectively recognizes polymorphonuclear cells.
LL37 / Cathelicidin Antibody - Sheep antibody to LL-37. WB on human neutrophil lysate using Sheep antibody to human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): IgG at 50 ug/ml concentration. Pre-absorption of the antibody with the immunizing peptide completely abolishes the detected band.
LL37 / Cathelicidin Antibody - Sheep antibody to LL-37. Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffy coat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Sheep antibody to human Cathelicidin antimicrobial peptide: IgG diluted 50 ug/ml: in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI. The antibody selectively recognizes polymorphonuclear cells.
LL37 / Cathelicidin Antibody - Sheep antibody to LL-37. Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffy coat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Sheep antibody to human Cathelicidin antimicrobial peptide: IgG diluted 50 ug/ml: in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI. The antibody selectively recognizes polymorphonuclear cells.
LL37 / Cathelicidin Antibody - Sheep antibody to LL-37. WB on human neutrophil lysate using Sheep antibody to human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39): IgG at 50 ug/ml concentration. Pre-absorption of the antibody with the immunizing peptide completely abolishes the detected band.
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Polyclonal Sheep anti‑Human LL37 / Cathelicidin Antibody (IF, WB) LS‑C93988

Polyclonal Sheep anti‑Human LL37 / Cathelicidin Antibody (IF, WB) LS‑C93988

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Antibody:
LL37 / Cathelicidin Sheep anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C93988-500
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
LL37 / Cathelicidin Sheep anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Cathelicidin antibody LS-C93988 is an unconjugated sheep polyclonal antibody to human Cathelicidin (LL37). Validated for IF and WB.
Target
Human LL37 / Cathelicidin
Synonyms
CAMP | Antibacterial protein fall-39 | CAP-18 | CRAMP | FALL-39 | FALL39 | HCAP-18 | HSD26 | LL37 | CAP18
Host
Sheep
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
Synthetic peptide as a part of human Cathelicidin antimicrobial peptide (CAP-18, hCAP-18, antibacterial protein LL-37, CAMP, CRAMP, FALL39) conjugated to an immunogenic carrier protein has been used as the immunogen.
Specificity
Specific for Cathelicidin antimicrobial peptide.
Applications
  • Immunofluorescence
  • Western blot
Usage
IHC: Antigen retrieval is essential for use on paraffin sections.
Presentation
Lyophilized
Reconstitution
500 µl Sterile water
Storage
Maintain lyophilized and reconstituted antibodies at -20°C for long term storage and at 2°C to 8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LL37 / Cathelicidin
P49913 NM_004345 NP_004336.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
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