Target
Human LIPG / Endothelial Lipase
Synonyms
LIPG | EDL | EL | Lipoprotein lipase H | Lipase, endothelial | PRO719 | Endothelial lipase
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-RFNLRTSKDPEHEG, from the internal region of the protein sequence according to NP_006024.1.
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx. 70kD band observed in Human Liver lysates (calculated MW of 56.8kD according to NP_006024.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gauster et al, J Clin Endocrinol Metab. 2007 Jun;92(6):2256-63. Epub 2007 Mar 13.; PMID: 17356047). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LIPG / Endothelial Lipase