Rat LILRA5

Goat

Rat (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

NS0-derived recombinant rat LILRC1 (aa17-248).

aa17-248

Selected for its ability to recognize rat LILRC1 in the applications listed.

• ICC (2 - 15 µg/ml)
• Western blot (0.1 - 0.2 µg/ml)
• Flow Cytometry
• ELISA (0.5 - 1 µg/ml)

Suitable for use in ELISA, Western Blot, Immunocytochemistry, Flow Cytometry. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect rat LILRC1. The detection limit for rrLILRC1 is approximately 1.5 ng/well. In this format, this antibody shows approximately 30% cross-reactivity with rmLILRCI and less than 1% cross-reactivity with rrLILRC2. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect rat LILRC1. The detection limit for rrLILRC1 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 2-15 ug/ml. This antibody will detect human rat LILRC1 in rat splenocytes. Flow Cytometry: Dilute to 50 ug/ml and add 10 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as anti-goat lgG conjugated to a fluorochrome. This antibody has been tested on rat splenocytes for use in flow cytometry.

Lyophilized

Reconstitute in deionized water

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: A6NI73 NCBI: NM_021250 NP_067073.1