Human LI2 / Abca12

Goat

Human, Monkey (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-KDQKSYETADTSSQ, from the internal regon (near C-Terminus) of the protein sequence according to NP_775099.2NP_056472.2.

aa2567-2580

Human ABCA12. This antibody is expected to recognize both reported isoforms (NP_775099.2; NP_056472.2).

• Peptide Enzyme-Linked Immunosorbent Assay (1:1000)

Peptide ELISA: antibody detection limit dilution 1:1000. Western Blot: Preliminary experiments gave an approx 35kD band in Human Placenta, Skin and Testis lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 193kD according to NP_775099.2 and of 257kD according to NP_056472.2. The 35kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q86UK0 NCBI: NM_015657 NP_056472.2