Target
Human LF / LTF / Lactoferrin
Synonyms
LTF | GIG12 | Growth-inhibiting protein 12 | HLF2 | Lactotransferrin | Lactoferrin | LF | Neutrophil lactoferrin | Talalactoferrin
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
FITC.
Also available
Unconjugated or conjugated with
Biotin,
HRP.
Purification
Ion exchange chromatography
Immunogen
Human Lactoferrin.
Specificity
Human LTF / Lactoferrin. In immunoelectrophoresis against human milk a single precipitin line is obtained. The antiserum does not react with any other protein component of human serum or plasma. Cross-reactivity The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however in double radial immunodiffusion a reaction with Rhesus monkey milk has been observed.
Applications
- Immunofluorescence
- ELISA
- Immunodiffusion
- Applications tested for the base form of this product only
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. This product is intended for use as reagent for the direct detection of lactoferrin in human cells, tissues and body fluids in immunofluores- cence; as detection reagent in non-isotopic methodology and solid phase immunochemistry (e. g. ELISA). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Use 1 ml of sterile Distilled water.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LF / LTF / Lactoferrin