Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Location


Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers



Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C59234-2 2 ml (82 mg/ml) $1,225 
LDH / Lactate Dehydrogenase Antibody - Lactate Dehydrogenase Antibody-Immunofluorescence. Biotin conjugated anti-lactate dehydrogenase antibody was used to stain HeLa cells by immunofluorescence. HeLa cells were plated in 12 well plates, fixed for 5 min in 1:1 Methanol: Acetone, blocked with MB-071 (preservative free) for 15 min and stained 1 hr with antibody 1:200 in blocking buffer. Plate was washed 3X in PBS, and primary biotin conjugate was detected by DyLight 488 conjugated Streptavidin (S000-41 lot 21097) 1:10000 for 30 min. Well was washed 3X in PBS.
LDH / Lactate Dehydrogenase Antibody - Anti-Lactate Dehydrogenase Antibody - Western Blot. Western blot analysis shows Anti-Lactate Dehydrogenase antibody detects LDH in HeLa cell extracts. Reactivity with LDH is observed in the cytoplasmic fraction (CF) and little to no reactivity in the nuclear fraction (NF). ~30 ug was loaded per lane onto a 10% gel for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kD corresponding to the expected molecular weight for the protein. The blot was incubated with a 1:1000 dilution of the antibody in 5% milk in TBST at room temperature followed by detection using standard procedures. Personal communication Kuldeep Patel.
LDH / Lactate Dehydrogenase Antibody - Lactate Dehydrogenase Antibody-Immunofluorescence. Biotin conjugated anti-lactate dehydrogenase antibody was used to stain HeLa cells by immunofluorescence. HeLa cells were plated in 12 well plates, fixed for 5 min in 1:1 Methanol: Acetone, blocked with MB-071 (preservative free) for 15 min and stained 1 hr with antibody 1:200 in blocking buffer. Plate was washed 3X in PBS, and primary biotin conjugate was detected by DyLight 488 conjugated Streptavidin (S000-41 lot 21097) 1:10000 for 30 min. Well was washed 3X in PBS.
LDH / Lactate Dehydrogenase Antibody - Anti-Lactate Dehydrogenase Antibody - Western Blot. Western blot analysis shows Anti-Lactate Dehydrogenase antibody detects LDH in HeLa cell extracts. Reactivity with LDH is observed in the cytoplasmic fraction (CF) and little to no reactivity in the nuclear fraction (NF). ~30 ug was loaded per lane onto a 10% gel for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kD corresponding to the expected molecular weight for the protein. The blot was incubated with a 1:1000 dilution of the antibody in 5% milk in TBST at room temperature followed by detection using standard procedures. Personal communication Kuldeep Patel.
1 of 2
2 of 2

Polyclonal Goat anti‑Rabbit LDH / Lactate Dehydrogenase Antibody (IF, WB) LS‑C59234

Polyclonal Goat anti‑Rabbit LDH / Lactate Dehydrogenase Antibody (IF, WB) LS‑C59234

Note: This antibody replaces LS-C25046
Antibody:
LDH / Lactate Dehydrogenase Goat anti-Rabbit Polyclonal Antibody
Application:
IF, WB, IP, ELISA
Reactivity:
Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$1,225
LS-C59234-2
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
LDH / Lactate Dehydrogenase Goat anti-Rabbit Polyclonal Antibody
Application:
IF, WB, IP, ELISA
Reactivity:
Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
Lactate Dehydrogenase antibody LS-C59234 is an unconjugated goat polyclonal antibody to rabbit Lactate Dehydrogenase (LDH). Validated for ELISA, IF, IP and WB.
Target
Rabbit LDH / Lactate Dehydrogenase
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
This antibody was prepared from whole goat serum produced by repeated immunizations with a full length lactate dehydrogenase protein isolated from rabbit muscle.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.
Applications
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:100)
  • ELISA (1:4000 - 1:20000)
Usage
This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 36 kD in size corresponding to LDH western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 2 mL of deionized water.
Storage
Store at 4°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/22/2024