Target
Rabbit LDH / Lactate Dehydrogenase
Reactivity
Rabbit
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Immunogen
This antibody was prepared from whole goat serum produced by repeated immunizations with a full length lactate dehydrogenase protein isolated from rabbit muscle.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.
Applications
- Immunofluorescence
- Western blot (1:500 - 1:2000)
- Immunoprecipitation (1:100)
- ELISA (1:4000 - 1:20000)
Usage
This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 36 kD in size corresponding to LDH western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 2 mL of deionized water.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee