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Catalog Number	Size	Price
LS-C783540-100	100 µg (0.5 mg/ml)	$285

LAMP1 / CD107a Antibody - Formalin-fixed paraffin-embedded human tonsil treated with a citrate buffer pH6 and heat for antigen retrieval was stained with purified CD107a clone H4A3, conjugated and detected with a Cy5 conjugated CODEX™ oligonucleotide duplex (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.

LAMP1 / CD107a Antibody - ICC staining of purified anti-LAMP-1 antibody (clone H4A3) on Hela cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then stained with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Cells were counterstained with Phalloidin™ Green 488 and DAPI to visualize actin filaments and nuclei, respectively. The images were captured with a 60X objective. Scale bar: 50 µm.

LAMP1 / CD107a Antibody - Western blot of purified anti-LAMP-1 antibody (clone H4A3). Lane 1: Molecular weight marker; Lane 2: 20 µg of Hela cell lysate; Lane 3: 20 µg of NIH3T3 cell lysate; Lane 4: 20 µg of human brain lysate; Lane 5: 20 µg of mouse brain lysate; Lane 6: 10 ng of human recombinant LAMP-1. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG. Anti-ß-actin antibody was used as the loading control. Enhanced chemiluminescence was used as the detection system.

LAMP1 / CD107a Antibody - Thrombin-activated human peripheral blood platelets were stained with purified CD107a (clone H4A3) (red histogram) or purified mouse IgG1, kappa (green histogram), followed by anti-mouse IgG FITC.

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Monoclonal Mouse anti‑Human LAMP1 / CD107a Antibody (clone H4A3, IHC, WB) LS‑C783540

Name: Anti-LAMP1 / CD107a Antibody | Mouse anti-Human Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C783540-100
Price: 285.0000 USD
Availability: InStock

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Antibody:

LAMP1 / CD107a Mouse anti-Human Monoclonal (H4A3) Antibody

Application:

IHC-P, ICC, WB, IP, Flo

Reactivity:

Human, Chimpanzee, Baboon, African green monkey, Cynomolgus monkey, Pig-tailed macaque, Rhesus monkey

Format:

Unconjugated, Unmodified

$285

LS-C783540-100

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Overview

Antibody:

LAMP1 / CD107a Mouse anti-Human Monoclonal (H4A3) Antibody

Application:

IHC-P, ICC, WB, IP, Flo

Reactivity:

Human, Chimpanzee, Baboon, African green monkey, Cynomolgus monkey, Pig-tailed macaque, Rhesus monkey

Format:

Unconjugated, Unmodified

Specifications

Description

CD107a antibody LS-C783540 is an unconjugated mouse monoclonal antibody to CD107a (LAMP1) from human. It is reactive with human, african green monkey, baboon and other species. Validated for Flow, ICC, IHC, IP and WB.

Target

Human LAMP1 / CD107a

Synonyms

See All LAMP1 / CD107a Antibodies

Host

Mouse

Reactivity

Human, Chimpanzee, Baboon, African green monkey, Cynomolgus monkey, Pig-tailed macaque, Rhesus monkey (tested or 100% immunogen sequence identity)

Clonality

IgG1,k Monoclonal

Clone

H4A3

Conjugations

Unconjugated

Purification

Purified by affinity chromatography.

Modifications

Unmodified

Immunogen

Human adult adherent peripheral blood cells

Applications

IHC - Paraffin (5 - 10 µg/ml)
ICC
Western blot (1 - 5 µg/ml)
Immunoprecipitation
Flow Cytometry (2 µg/10E6 cells)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 2.0 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunocytochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg per ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining, immunofluorescence, and immunoprecipitation.This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Presentation

PBS pH 7.2, 0.09% sodium azide.

Storage

Store at 2°C to 8°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About LAMP1 / CD107a

Uniprot: P11279 NCBI: NM_005561 NP_005552.3