Human Laminin

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Biotin

Affinity purified

Unmodified

Non reduced human laminin (400kD).

Typically less than 1% cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. Recognizes human Laminin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross-reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.

• IHC
• IHC - Paraffin
• Western blot (1:2500 - 1:5000)
• Immunoprecipitation
• ELISA (1:2000 - 1:4000)

Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry, and Western Blot. ELISA: 1:2000-1:4000. 1 ug of Laminin in aa standard capture ELISA with Streptavidin and ABTS as a substrate for 30 minutes at RT. Western Blot: 1:2500-1:5000. Immunohistochemistry (Paraffin): 1:25-1:100. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.