Human KRT9 / CK9 / Cytokeratin 9

Mouse

Human, Mouse (tested or 100% immunogen sequence identity)

IgG1 + IgG3 Monoclonal

Unconjugated

Tissue culture supernatant

Unmodified

Synthetic peptides [(1) aa450-477 and (2) N-Terminus aa4-28] of human cytokeratin 9 (MW 62129).

aa450-477

Recognizes human Cytokeratin 9 (in mouse: some background staining). Antibody represents an excellent marker to study palmoplantar epidermal distribution and differentiation. Specifically reactive in the middle/upper suprabasal layers (stratum spinosum/ granulosum) of the epidermis of palm and sole. No labeling has been found in epithelial cells of other stratified epithelia such as esophagus or complex epithelia (e. g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum.

• IHC
• IHC - Paraffin (1:20)
• IHC - Frozen (1:20)
• Immunofluorescence (1:10)
• Western blot (1:25 - 1:300)

Suitable for use in Western Blot (Western), Immunofluorescence and Immunohistochemistry (frozen tissue, for paraffin-embedded sections, microwave treatment and in some cases protease digestion is recommended). The antibody cocktail is also an excellent new tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment. Western Blot: 1:25-1:300 (chemiluminescence, AP/ECL). Immunofluorescence Microscopy: 1:10. Immunohistochemistry: 1:20 (PAP/ABC).

0.09% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P35527 NCBI: NM_000226 NP_000217.2