Human KRT7 / CK7 / Cytokeratin 7

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Recombinant Monoclonal

rOV-TL12/30

Unconjugated

Protein A/G purified

Azide-free, Carrier-free. Also available Unmodified.

Recombinant full-length human KRT7 protein. Antigen Molecular Weight: 55 kDa

It recognizes an intermediate filament protein (IFP) of 55 kDa , which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

• IHC - Paraffin (0.5 - 1 µg/ml)
• Western blot (1 - 2 µg/ml)
• Flow Cytometry (1 - 2 µg/10E6 cells)

Immunohistology (Formalin-fixed - 0.5-1.0 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

10 mM PBS

Store at -20°C to -80°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

NCBI: NP_005547.3