Human KRT6 / CK6 / Cytokeratin 6

Mouse

Human, Horse, Sheep (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

SPM269

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified.

Synthetic peptide from human keratin 6 (GSSTIKYTTTS, aa545-555 of UniProt P48668). Percent identity by BLAST analysis: Human, Sheep, Horse (100%); Mouse, Rat, Ferret, Cat, Bat, Hamster, Elephant, Dog (91%); Bovine, Pig, Opossum (82%).

aa545-555

Human KRT6 / CK6 / Cytokeratin 6

• IHC
• IHC - Paraffin (0.1 - 0.2 µg/ml)
• Immunofluorescence (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation. The optimal dilution of the Cytokeratin 6 antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.