Target
Human KRT2 / CK2 / Cytokeratin 2
Synonyms
KRT2 | Cytokeratin-2e | CK-2e | Epithelial keratin-2e | K2e | Keratin 2 | Keratin-2e | Krt2e | KRT2A | KRTE | Keratin-2 epidermis | Type-II keratin Kb2
Reactivity
Human, Mouse, Rat, Bovine
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Immunogen
Synthetic peptide corresponding to the N-Terminus aa2-23 of human cytokeratin 2e (MW 65852).
Specificity
Reactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. Recognizes individual cells within epidermis of tongue and mamille (co-localization with cytokeratin 10). Reactive on hyperkeratosis of diverse viral and genetic origin. Does not cross-react with palate cytokeratin 2p. Species cross-reactivity: Human. Bovine: Positive with calf hoof epidermis, snooze epithelium. Negative with tongue epithelium. Mouse/rat: Heterogeneously positive with foot sole epidermis only. Negative with epidermis of other body sites.
Applications
- IHC
- IHC - Paraffin
- IHC - Frozen
- Immunofluorescence (1:200)
- Western blot (1:200)
- ELISA
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Usage
Suitable for use in ELISA, Western Blot, Immunohistochemistry. Excellent marker to study terminal epidermal differentiation. Immunohistochemistry: 1:100. Note: Paraffin sections require antigen retrieval with microwave. Frozen sections require antigen retrieval by pretreatment with PBS, 0.2% Triton-X100 for 5 minutes. Immunohistochemistry: 1:200 (PAP/ABC). Immunofluorescence: 1:200. Western Blot: 1:200 (alkaline phosphatase). Western Blot: 1:60000 (ECL).
Presentation
Tissue Culture Supernatant
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KRT2 / CK2 / Cytokeratin 2