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Catalog Number	Size	Price
LS-C352434-100	100 µl (1 mg/ml)	$299

KRT16 / CK16 / Cytokeratin 16 Antibody - Immunohistochemical analysis of Cytokeratin 16 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

KRT16 / CK16 / Cytokeratin 16 Antibody - Immunofluorescent analysis of Cytokeratin 16 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

KRT16 / CK16 / Cytokeratin 16 Antibody - Western blot analysis of Cytokeratin 16 expression in HeLa (A) whole cell lysates.

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Polyclonal Rabbit anti‑Human KRT16 / CK16 / Cytokeratin 16 Antibody (C‑Terminus, IHC, IF, WB) LS‑C352434

Name: Anti-KRT16 / CK16 / Cytokeratin 16 Antibody | Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-C352434-100
Price: 299.0000 USD
Availability: InStock

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Antibody:

KRT16 / CK16 / Cytokeratin 16 Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

$299

LS-C352434-100

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

KRT16 / CK16 / Cytokeratin 16 Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

Cytokeratin 16 antibody LS-C352434 is an unconjugated rabbit polyclonal antibody to human Cytokeratin 16 (KRT16 / CK16) (C-Terminus). Validated for ICC, IF, IHC and WB.

Target

Human KRT16 / CK16 / Cytokeratin 16

Synonyms

KRT16 | CK16 | Cytokeratin 16 | Cytokeratin-16 | FNEPPK | K1CP | Keratin-16 | Keratin 16 | NEPPK | CK-16 | K16 | KRT16A

See All KRT16 / CK16 / Cytokeratin 16 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human Cytokeratin 16.

Epitope

C-Terminus

Specificity

Recognizes endogenous levels of Cytokeratin 16 protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About KRT16 / CK16 / Cytokeratin 16

Uniprot: P08779 NCBI: NM_005557 NP_005548.2