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Catalog Number	Size	Price
LS-C352436-100	100 µl (1 mg/ml)	$299

KRT14 / CK14 / Cytokeratin 14 Antibody - Immunohistochemical analysis of Cytokeratin 14 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

KRT14 / CK14 / Cytokeratin 14 Antibody - Immunofluorescent analysis of Cytokeratin 14 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

KRT14 / CK14 / Cytokeratin 14 Antibody - Western blot analysis of Cytokeratin 14 expression in MCF7 (A); mouse brain (B); rat brain (C) whole cell lysates.

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Polyclonal Rabbit anti‑Human KRT14 / CK14 / Cytokeratin 14 Antibody (N‑Terminus, IHC, IF, WB) LS‑C352436

Name: Anti-KRT14 / CK14 / Cytokeratin 14 Antibody | Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-C352436-100
Price: 299.0000 USD
Availability: InStock

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Antibody:

KRT14 / CK14 / Cytokeratin 14 Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$299

LS-C352436-100

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For Research Use Only

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Overview

Antibody:

KRT14 / CK14 / Cytokeratin 14 Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

Cytokeratin 14 antibody LS-C352436 is an unconjugated rabbit polyclonal antibody to Cytokeratin 14 (KRT14 / CK14) (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.

Target

Human KRT14 / CK14 / Cytokeratin 14

Synonyms

KRT14 | CK-14 | EBS3 | EBS4 | K14 | Keratin-14 | CK14 | Cytokeratin 14 | Cytokeratin-14 | Keratin 14 | NFJ

See All KRT14 / CK14 / Cytokeratin 14 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human Cytokeratin 14.

Epitope

N-Terminus

Specificity

Recognizes endogenous levels of Cytokeratin 14 protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About KRT14 / CK14 / Cytokeratin 14

Uniprot: P02533 NCBI: NM_000526 NP_000517.2