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Antibodies > KIAA0652 / ATG13 > LS-C482241
 
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Catalog Number Size Price
LS-C482241-50 50 µl (1 mg/ml) $286 
LS-C482241-100 100 µl (1 mg/ml) $321 
LS-C482241-200 200 µl (1 mg/ml) $386 
KIAA0652 / ATG13 Antibody - Immunofluorescent analysis of ATG13 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
KIAA0652 / ATG13 Antibody - Western blot analysis of ATG13 expression in T47D (A); SW480 (B) whole cell lysates.
KIAA0652 / ATG13 Antibody - Immunofluorescent analysis of ATG13 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
KIAA0652 / ATG13 Antibody - Western blot analysis of ATG13 expression in T47D (A); SW480 (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human KIAA0652 / ATG13 Antibody (IF, WB) LS‑C482241

Polyclonal Rabbit anti‑Human KIAA0652 / ATG13 Antibody (IF, WB) LS‑C482241

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Antibody:
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$286
LS-C482241-50
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ATG13 antibody LS-C482241 is an unconjugated rabbit polyclonal antibody to ATG13 (KIAA0652) from human. It is reactive with human, mouse and rat. Validated for ICC, IF and WB.
Target
Human KIAA0652 / ATG13
Synonyms
ATG13 | Autophagy-related protein 13 | KIAA0652 | Autophagy related 13
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Recombinant full length human ATG13.
Specificity
Recognizes endogenous levels of ATG13 protein.
Applications
  • ICC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KIAA0652 / ATG13
O75143 NM_014741 NP_001136145.1

Publications (0)

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Featured Products

KIAA0652 / ATG13 Antibody - Anti-ATG13 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ KIAA0652 / ATG13 Antibody LS‑B5277
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
KIAA0652 / ATG13 Antibody - Western blot of affinity purified anti-ATG13 pS318 antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13). In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto (p/n B501-0500) and probed with the primary antibody at 1g/mL overnight at 4C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer (p/n MB-070) for 45 minutes at room temperature. In collaboration with Charles Dorsey at Eli Lilly, Indianapolis, IN and John Cleveland at Scripps, Jupiter, FL.
KIAA0652 / ATG13 Antibody (phospho‑Ser318) LS‑C153855
Species: Human
Applications: Western blot, Flow Cytometry, ELISA
KIAA0652 / ATG13 Antibody - Western blot using the affinity purified anti-ATG13 pS318 antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13). In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto and probed with the primary antibody at 1µg/mL overnight at 4°C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer for 45 minutes at room temperature.
KIAA0652 / ATG13 Antibody (phospho‑Ser318) LS‑C745344
Species: Human
Applications: Western blot, Flow Cytometry, ELISA
KIAA0652 / ATG13 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG13 Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG13 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm, Cytosol. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG13 Antibody (D) Composite.
KIAA0652 / ATG13 Antibody (Biotin) LS‑C773977
Species: Human, Mouse
Applications: Immunofluorescence, Western blot
KIAA0652 / ATG13 Antibody - Immunohistochemistry of paraffin-embedded rat brain using ATG13 antibodyat dilution of 1:100 (40x lens).
KIAA0652 / ATG13 Antibody LS‑C331070
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
See all KIAA0652 / ATG13 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
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